Microscale Thermophoresis (NanoTemper Monolith NT.115 Blue/Red)

The MST platform is designed to quickly and easily quantitate interaction strength between biomolecules, be they proteins, small peptides, cofoactors, or ions. This powerful technique requires exceedingly small quantities of sample (approximately 60 μL total) in concentration ranges that are typical of bioanalysis.

There is no immobilization chemistry required to complete a study, and sample preparation via fluorophore labeling is straightforward. Interactions may be investigated by MST in a wide array of buffers ranging from standard physiological conditions to complex formulations with high detergent content and even in cell lysate, thus obviating the need for tedious purification as required by other methods.